ROSE BENGAL STAINING Rose bengal is another stain which stains epithelial cells (on the cornea or conjunctiva) where the mucoid layer of the tear film is missing, dead or degenerating cells, and mucous strands. It is supplied on strips just like fluorescein or as a solution. Only a small amount of the stain is required and if too much is instilled it will be uncomfortable for the patient. One must be careful with it as it will stain clothing and not be easily washed out as fluorescein is. Areas of staining will appear as reddish-purple spots.